The Timing of NO expression in brain tissue after diffused traumatic brain injury / 中华急诊医学杂志

ABSTRACT

Object　To determine the timing of NO expression in brain tissue after severely diffused brain injury．It would contribute to the understanding of the pathological and physiological functions of NO．Methods　On the basis of the Marmarou diffused brain injury model，the rat was killed at different hours afterward．and the NO expression in brain was measured by Griess reaction．Results　It was shown that NO in brain tissue increased quickly after trauma （5.20umol／100g），then decreased in 12h（1.96umol／100g）after　trauma，a secondary NO elevation appeared in 24h（2.31umol／100g） and 48h（2.69umol／100g） after DBI．Conclusion　It indicated that NO expression in brain tissue increased after trauma．As MCAO ischemia model，there was also NO increase at the intermediate and late stage，which might take part in the secondary pathological changes at late stage of cerebral injury．