Citrus fiavonoids simultaneous HPLC analysis / 中成药
Chinese Traditional Patent Medicine
;
(12): 99-104, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-408115
ABSTRACT
AIM:
To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids, naringin, hesperidin, neohesperidin, sinensetin and nobiletin.METHODS:
Gradient elution with non-salt mobile phase ( methanol and water only) HPLC method on a Kromasil column ( 100-5C18-250A, 4.6 mm ×250 mm, 5 μm, C18 reverse phase) with peaks identification through DAD full UV wavelength scan. UV 284 nm and 332 nm profiles were observed.RESULTS:
Satisfactory resolution, linearity, 95%~ 102% of recovery and 1.88 ~ 2.93% of repeatability were obtained for those five citrus flavonoids. Content of 6 Citrus aurantium L. based TCM ingredients were analyzed and identified.CONCLUSION:
Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2007
Type:
Article
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