Detection of anti-glutamic acid receptor 1 antibody in serum of epilepsy children

Changqian ZENG; Lijuan SU; Jinyan WANG

ABSTRACT

BACKGROUND:

The recent researches show that there are various kinds of autoantibodies in epilepsy patients such as anti-nuclear antibodies, anticardiolipin antibodies,anti-?2 glucoprotein antibodies and anti-glutamic acid decarboxylase antibodies,which indicate that some types of epilepsy are mediated by autoimmune mechanism.

OBJECTIVE:

To detect the level of anti-glutamic acid receptor (GluR1)antibody in epilepsy children.

DESIGN:

A controlled trial.

SETTING:

Medical College of Dalian University, General Hospital of Jilin Chemistry Group and Sanchong University, Japan.

PARTICIPANTS:

Totally 56 cases of epilepsy children were from Maternal and Child Health in Qingdao City including 30 males and 26 females and aged from 3 months to 13 years.While 48 health examinees and 12 patients with brain tumor from General Hospital of Jilin Chemistry Group were taken as controls, including 38 males and 12 females and aged from 6months to 17 years.Their guardians were all informed of the detection index and consented to join the study.

METHODS:

Polystyrene plates were coated with GluR1 polypeptide antigen,and optimal conditions of enzyme linked immunosorbent assay (ELISA) for GluRI antibody was ascertained with chessboard titration.MAIN OUTCOME

MEASURES:

The GluR1 antibody level was detected with ELISA.

RESULTS:

The concentration of coating GluR1 polypeptide antigen was20 mg/L; The dilution of serum and horseradish peroxidase sheep anti-hu-man (HRP-SaH) IgG were 150 and 11 500 respectively. Intra-assay andinter-assay coefficientof variability were 4.48％-8.80％ and 11.18％-16.60％ respectively; The specific absorption test demonstrated that the ab-sorbance value of positive serum decreased 2.4 times after absorption ofGluR1 polypeptide compared with that before absorption.There was obvi-ous higher positive rate of GluR1 antibody in serum of epilepsy childrenthan control group (40％, 5％, P＜0.01).

CONCLUSION:

①ELISA is specific and stable for GluR1 antibody in serum. ②There is the possible autoimmune response of GluR1 antibody in serum of epilepsy children.