Effect of Ca2+-almodulin on contraction of M3R-mediated detrusor muscle cell

Gensheng LU; Zhansong ZHOU; Zhiwen CHEN; Bo SONG; Weibing LI; Enqing XIONG

ABSTRACT

BACKGROUND:

Muscarine receptor plays a key role in adjusting contraction of detrusor muscle cell, and M3R, isoforms of its receptor, can mediate contraction of detrusor muscle cell directly. Ca2+ is the direct factor in stimulating contraction of detrusor muscle cell. Of several 10 kinds of Ca2+conjugable receptors protein, Ca2+ conjugated with different receptor proteins can adjust various reactions.

OBJECTIVE:

To investigate the effect of Ca2+-calmodulin (Ca2+-CaM) on contraction of M3R-mediated detrusor muscle cell.

DESIGN:

Compared observation .on the basis of detrusor muscle cell.

SETTING:

Ourological Center of Southwest Hospital of the Third Military Medical University of Chinese PLA.MATERIALS The experiment was completed at Central Laboratory of Southwest Hospital of the Third Military Medical University of Chinese PLA. Healthy Wistar rats were selected in this study.

METHODS:

The primary cultured detrusor muscle cells were divided into experimental group and control group. Cells were inoculated in 6-well plate, and 10-4 mmol/L carbachol and M2R antagonist were added to cells of the experimental group during 70% confluence to block M3R and M2R respectively. Ca2+ concentration and CaM activity were detected by Ca2+ test kit and CaM test kit respectively.MAIN OUTCOME

MEASURES:

Changes of [Ca2+]I concentration and CaM activity of cells in both groups.

RESULTS:

The mean channel fluorescence values (log) of [Ca2+]I and CaM were higher in the experimental group than those in the control group(3.26±0.38, 2.06±0.12; 2.87±0.34, 2.14±0.24, P ＜ 0.05).

CONCLUSION:

Results in this study suggest that Ca2+-CaM participates in adjusting contraction of M3R-mediated detrusor muscle cells through signal transduction.