Effect of two modified compound preparations of wuling san on blood brain barrier

Tai LIU; Yuping TANG; Xiangfa ZENG; Jing Lü

ABSTRACT

BACKGROUND:

On the basis of traditional herb wuling san, tianqi and danshen are added to make a preparation, jianshen lishui I , for treating blood stasis and edema; shichangpu and xiakucao are added to make a preparation, xingnao xiaozhong capsule, for treating fire, sputum and qiaobi in cerebral edema.

OBJECTIVE:

To observe the differences between jianshen lishui Ⅰ and xingano xiaozhong capsule on treating vascular-derived cerebral edema.

DESIGN:

Randomized grouping design and blank controlled study.

SETTING:

Laboratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine.MATERIALS The experiment was completed in the Lab.oratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine from March to April 2001. Totally 32 male rabbits with 7 months old were selected in the study.

METHODS:

① Animal models with cerebral edema were established with self-fresh blood injection method. According to grouping method, animals were divided randomly into 4 groups blank control group (without intervention); model group (without intervention after modeling); jianshen lishuiⅠ group (11.5 mL/kgjianshen lishui Ⅰ was perfused after modeling; jian-shen lishui Ⅰ consisted of 15 g sanqi, 15 g danshen, 10 gfuling, 10 gzhuling, 10 g zexie, 10 g baizhu and 10 g gu~hi, and provided by Dispensary of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine, 200 mL/bag) and xingnao xiaozhong capsule group [after modeling, 11.5 mL/kg xingnao xiaozhong suspension was made by 380 mg/kg boiled distilled water; certification southern medicine (96) 05079; consisting of 15g xiakucao, 15 g shichangpu, 10 gfuling, 10 g zhuling, 10 g zexie, 10 g baizhu and 10 g guizhi, 13.33 g raw materials per capsule; provided by Agent Department of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine] with 8 in each group. Animals in each group were divided into 12 and 24 hours subgroups with 4 in each time points. ② Animals were killed 12 and 24 hours after intervention with air embolism to calculate cerebral coefficient (total brain mass/body mass × 100%), cerebral water content [(wet weight - dry weight)/wet weight × 100%], Evens blue content in brain tissue [absorbency (A value) × microgram in standard liquid/an A value × (25 mL/wet weight)]. ③Positivity was tested with square method; differences among groups were compared with F test; differences between groups were compared with q test; time points were tested with t test; and irregular variance was checked with rank sum test.MAIN OUTCOME

MEASURES:

Comparisons of cerebral coefficient,water content and content of Evans blue in 12 and 24-hour experiment group.

RESULTS:

Totally 32 rabbits entered the final analysis. ① 12-hour experiment Water content and Evans blue content in jianshen lishui Ⅰgroup and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01); cerebral coefficient in jianshen lishui I group was lower than that in xingnao xiaozhong capsule group, but the difference was not significant (P ＞ 0.05); water content and Evans blue content in jianshen lishui Ⅰ group were lower than those in xingnao xiaozhong capsule group (P ＜ 0.05). ② 24-hour experiment Water content and Evans blue content in jianshen lishui Ⅰ group and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01);cerebral coefficient in jianshen lishui Ⅰ group was lower than that in model group (P ＜ 0.01); cerebral coefficient, water content and Evans blue content in jianshen lishui Ⅰ group were respectively lower than those in xingnao xiaozhong capsule group (P ＜ 0.05).

CONCLUSION:

Vascular-derived edema has found 12 hours after modeling and cerebral edema deteriorates with time passing. In addition, effect of jianshen lishui Ⅰ on blood brain barrier is superior to that of xingnao xiaozhong capsule.