Application of microgravity bioreactor in cell culture / 中国组织工程研究

ABSTRACT

OBJECTIVE: Microgravity culture is an emerging cultural technology,which can be used in many fields such as tissue engineering and cell culture,etc. Microgravity bioreactor was used for cell culture in simulated microgravity environment.DATA SOURCE We search the PubMed database from January 1995 to June 2002, using the key words "microgravity,simulated microgravity,bioreactor, tissue engineering, cell culture, rotating cell culture system" and the language was confined to English.STUDY SELECTION Inclusion criteria ① Research of cell culture in simulated microgravity condition. ② Research of tissue engineering of microgravity. ③ Randomized controlled empirical study. ④ Establishment of the simulated microgravity environment. Exclusion criteria ①Quite obsolete literatures. ② Repeated research.DATA EXTRACTION A total of 288 articles on reparation of spinal cord injury were collected, according to the inclusion criteria 19 articles were included, and the other 269 articles were excluded because they were repetitive and duplicated researches.DATA SYNTHESIS: ①Cultivation in simulated microgravity had its particular feature and practicability Shearing force formed by the turbulent flow was avoided; Culture fluid was admixed by rotating; There exists Zero headspace; Anchorage-dependent cell could grow on the carriers; Sufficient oxygenation was provided. ② Cell growth in simulated microgravity environment cells were prone to aggregate, and much higher cell density was generated. ③ Three-dimensional culture system of animal cells was constructed with microgravity bioreactor to satisfy organ surrogate or requirement of investigation in vitro. ④ Angioid tubiform fabric was generated in the three-dimensional tissue structure constructed with microgravity bioreactor, which was used to generate the blood vessel pipage of tissue engineering. ⑤ Problems needing to solve Once separated from the microgravity environment, whether the function of three-dimensional structure constructed with microgravity bioreactor can be still remained for long term or not and the structure will be kept invariably or not after transplanted and integrated to parasitifer tissue need further research.CONCLUSION: Technique of simulated microgravity culture is a original tissue culture technology. The central technique is the construction of three-dimensional culture system of animal cells, which can provide a favourable cultural surrounding for the growth and metabolism of mutiplicate cells and tissue blocks, carry out the tissue culture of high density, and keep the specificity of histological differentiation of cultural cells.