Antioxidation of soybean isoflavone in vascular endothelial cells with oxidative damage / 中国组织工程研究

ABSTRACT

BACKGROUND: Soybean isoflavone has a variety of bioactivities and its antioxidation becomes a hot spot of research in recent years. At present,the research of soybean isoflavone places more emphasis on animal experiment and clinical observation,but lacks research on cellular level of human body.OBJECTIVE: To observe the influence of soybean isoflavone in vascular endothelial cells with oxidative damage.DESIGN: Controlled trial and observation.SETTING: Central Laboratory, Institute of Public Health,Tianjin Medical University.MATERIALS The experiment was completed in the Central Laboratory,Institute of Public Health,Tianjin Medical University from January to July 2002.The experimental materials included vascular endothelial cell strain in human umbilical vein,low density lipoprotein,soybean isoflavone and vitamin E,etc.METHODS: The vascular endothelial cells were cultured in vitro.The experiment was divided into 6 groups blank control group,oxidative damage control group (malondialdehyde content was 1 μmol/L),oxidative damage+vitamin E control group(vitamin E was 50 μmol/L) and oxidative damage+soybean isoflavone 10,50,100 μ mol/L control group. The endothelial cells,which were joined with vitamin E and soybean isoflavone of different concentrations in advance to be incubated for 24 hours,were affected by oxidized low density lipoproteins and then cultured continually for 24 hours.All the indexes of antioxidation were determined in both extra-cell and intra-cell.MAIN OUTCOME MEASURES: Malondialdehyde content,activity of superoxide dismutase and glutathione peroxidase,the release condition of lactate dehydrogenase and productive quantity of nitrogen monoxide inside and outside the endothelial cells of each group.RESULTS: ①Comparison of malondialdehyde content,the activity of superoxide dismutase and glutathione peroxidase in endothelial cells of each group The malondialdehyde content was higher significantly in oxidative damage control group than in blank control group (P ＜ 0.01),but the activity of superoxide dismutase and glutathione peroxidase was lower significantly in oxidative damage control group than in blank control group(P ＜ 0.01).The malondialdehyde content was lower significantly in oxidative damage+vitamin E control group,oxidative damage+soybean isoflavone 10,50,100 μmol/L control group than in oxidative damage control group(P ＜ 0.01),but the activity of superoxide dismutase and glutathione peroxidase was higher significantly in oxidative damage+vitamin E control group,oxidative damage+soybean isoflavone 10,50,100 μ mol/L control group than in oxidative damage control group (P ＜ 0.01). ②Comparison of the release condition of lactate dehydrogenase and the productive quantity of nitrogen monoxide in endothelial cells of each group The release percentage of lactate dehydrogenase was higher significantly in oxidative damage control group than in blank control group (P ＜ 0.01),but the productive quantity of nitrogen monoxide was lower significantly in oxidative damage control group than in blank control group(P ＜ 0.01).The release percentage of lactate dehydrogenase was lower significantly in oxidative damage+vitamin E control group,oxidative damage+soybean isoflavone 10,50,100 μmol/L control group than in oxidative damage control group (P ＜ 0.01),but the productive quantity of nitrogen monoxide was higher significantly in oxidative damage+vitamin E control group,oxidative damage+soybean isoflavone 10,50,100 μmol/L control group than in oxidative damage control group(P ＜ 0.01).CONCLUSION: Soybean isoflavone can alleviate the oxidative damage in vascular endothelial cells,caused by oxidized low density lipoprotein,possibly through such antioxidization indexes as malondialdehyde content,the activity of superoxide dismutase and glutathione peroxidase,the release condition of lactate dehydrogenase and the productive quantity of nitrogen monoxide,etc.