Effect of tongxinluo capsule on reperfusion myocardial apoptosis / 中国组织工程研究

ABSTRACT

BACKGROUND:The therapeutic effect of traditional Chinese drug on myocardial ischemia and reperfusion has been increasingly attached importance to. The tongxinluo, a Chinese paten drug taking insect medicine as its major gradients, has been achieved satisfactory effect in anti-myocardial ischemia clinicallyOBJECTIVE: To investigate the effect of tongxinluo on myocardial apoptosis induced by ischemic reperfusion.DESIGN:A randomized controlled trialSETTING: Department of Traditional Chinese Medicine (TCM) of the First Affiliated Hospital, Sun Yat-sen UniversityMATERIALSThe experiment was conducted from November to December 2002 at the Molecular Medicine Research Center of Sun Yat-sen University.METHODS:Totally 72 Kunming mice were at random divided into 6groups with 12 in each group 2 g/kg tongxinluo, 1.5 g/kg tongxinluo, 1.0g/kg tongxinluo group (The mice were administrated by gavage, once a day, on the day 5, one hour after administration, pituitrin and nitroglycerin were in combination used for building the myocardial ischemic reperfusion model), isosorbide dinitrade group (The dose was 3.9 g/kg, and the rest treatment was the same as the above), model group and blank control group(Normal saline was taken as substitute for the drug). At the end of trial the myocardia of the mice were taken for investigating the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of myocardia in mice with ischemic reperfusion, analyzing the myocardial apoptosis index, and determining the expression levels of apoptosis-relevant proteins bcl-2 and bax.MAIN OUTCOME MEASURES: [1] Comparison of SOD activity and MDA level in mice of each group. [2] Comparison of myocardial apoptosis and apoptosis relevant proteins.RESULTS:Totally 72 mice involved were good for the trial and no one died. [1] SOD activity and MDA level Compared with the model and isosorbide dinitrade groups, SOD activity in 2 g/kg tongxinluo, 1.5 g/kg tongxinluo, 1.0 g/kg tongxinluo group was increased [( 1379.28±140.03 ),(1223.91 ±109.02), (1789.19 ±155.20), (2267.79 ±216.04), (2387.55±229.71 )nkat/L]; MDA level was decreased [(16.58±2.59), (20.40±2.66),(12.73±1.98), (9.65±2.03), (7.56±1.67)nmol/L], and the effect was increased as the dose was raised [2] myocardial apoptosis and apoptosis relevant proteins Compared with the model and isosorbide dinitrade groups, the myocardial apoptosis index and Bax expression index in 2 g/kg tongxinluo,1.5 g/kg tongxinluo, 1.0 g/kg tongxinluo group were obviously decreased[(13.84±1.97)%, (11.56±1.43 )%, ( 334±0.96)%, (0.82±0.12 )%, (0.42±0.06)%;(17.44±6.18)%, (15.45±3.72)%, (10.85±2.73)%, (6.46±1.88)%, (5.57±1.49)% ]; Bcl-2 expression index was obviously increased [(6.22±0.50)%, (8.73 ±0.63)%, (11.38 ±1.38)%, (16.22 ±2.36)%, (19.45±2.92)%], and with a dose-effect relationship.CONCLUSION:Tangible capsule had the effects of. resisting oxygen free radical injury, anti-myocardial apoptosis and protecting ischemic reperfusion myocardial cells, and with a dose dependence.