The biologic effect of fibrin-binding synthetic oligopeptide on periodontal ligament cells / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 45-52, 2009.
Article
in Korean
| WPRIM
| ID: wpr-40949
ABSTRACT
PURPOSE:
Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells. MATERIALS ANDMETHODS:
PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed.RESULTS:
PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes.CONCLUSION:
These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Peptides
/
Periodontal Ligament
/
Phosphorylation
/
Binding Sites
/
Fibrin
/
Cell Adhesion
/
Blotting, Western
/
Fibronectins
/
Ligaments
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2009
Type:
Article
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