Determination of amodiaquine in human plasma by high performance liquid chromatography / 中国新药与临床杂志
Chinese Journal of New Drugs and Clinical Remedies
;
(12): 925-928, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-409596
ABSTRACT
AIM:
To establish HPLC method for the determination of amodiaquine in human plasma.METHODS:
Amodiaquine and internal standard(hydroxychloroquine) were analyzed on C18 column(150 mm ×4.6 mm, 5 μm) with methanol watertriethylamine orthophosphoric acid (2177.510.5 )as mobile phase at the flow rate of 1.0 mL · min-1The UV detector was set at 294 nm.RESULTS:
The retention times of amodiaquine and internal standard were 5.82, 8.56 min, respectively. The calibration curve was linear in the range from 10 to 1 000 μg ·L- 1 ( r = 0.999 8, n = 9 ). The limit of quantitation was5 μg· L-1. The extraction recovery was between 75.5 % and 82.7 %, and the methodological recovery was between 97.0 % and 104.8 %. The intra-day and inter-day RSD were less than 6.0 % and 7.5 %, respectively.CONCLUSION:
This HPLC method is simple, sensitive and suitable for pharmacokinetic study of amodiaquine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of New Drugs and Clinical Remedies
Year:
2005
Type:
Article
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