Increasing the Production of IgG in Batch Tissue-culture with IgG-free Ascites / 中国生物工程杂志
China Biotechnology
;
(12): 87-92, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-409670
ABSTRACT
An effective method has been developed for laboratory scale production of IgG. Hybridomas were cultured in serum-free media with 2% IgG-free ascites. Cell density of up to 3.55 × 10 6cells/ml and antibody concentration of 135μ g/ml after purification were abtained, which is four time more than total production of that of IgG concentration in serum-free media. This in vitro method allows great improvement in antibodies production in batch tissue culture. The method reported here is easy to handle and is economical and universally adaptable.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Biotechnology
Year:
2005
Type:
Article
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