Effect of angong niuhuang wan on serum lactate dehydrogenase isoenzymes in rats with cerebral infarction

Yishan TANG; Xinhua PAN; Peixun WANG; Ningsheng WANG

ABSTRACT

BACKGROUND:

Angong niuhuang wan consists of mercury and arsenic, which has drawn the attention on its safety. It is necessary to carry on the research on evaluation in its availability and safety.

OBJECTIVE:

To study the mechanic differences of angong niuhuang wan in the organic body physiologically and pathologically.

DESIGN:

Randomized controlled experiment based on the experimental animals.

SETTING:

Institute of clinical pharmacology affiliated to a university.MATERIALS The experiment was performed in Institute of Clinical Phar macology affiliated to Guangzhou University of Traditional Chinese Medicine from March to April in 2001. Guangdong Medical Experimental Animal Center provided 24 SD male rats, weighted varied from 250 g to 300 g.

METHODS:

SD rats were randomized into 4 groups, named as normal group, normal & angong niuhuang wan group(normal & wan group), model of cerebral infarction group(model group) (The middle cerebral artery embolism was induced in rats by photochemistry. ) and model of cerebral in farction group & angong niuhuang wan group (model & wan group), 6 rats in each group. Medication instruction gastric feeding was applied once daily, 0. 13 g/kg, totally for 7 days. MAIN OUTCOME

MEASURES:

contents of lactate dehydrogenase (LDH) isoenzymes, LDH1-5.

RESULTS:

Contents of serum LDH1-3 in normal & wan group were significantly increased than those in normal group( P ＜ 0.01 ), of which, the values of LDH1, LDH2, LDH3 were(17.02 ±0.46)%, (14.70 ±0. 18)%,(15.47±0.13)% successively in normal & wan group, were(11.25 ± 0. 70) %, (8.26 ± 0. 90) %, ( 12. 86 ± 0.90) % successively in normal group. Content of serum LDH3[(15.51 ±2.60)% [ in model & wan group was significantly increased compared with that in model group[(10.93±2.10)% ] (P ＜ 0.01) . The contents of LDH4 were[(22.62 ±3.00)% ] and(28. 18 ±0. 80)% respectively in two groups, indicating significantly reducing( P ＜ 0.01 ).

CONCLUSION:

The organic injury of angong niuhuang wan is less in pathological state of focal cerebral infarction than that in physiological state,suggesting that the mechanic differences of angong niuhuang wan in organic body present in physiological and pathological states.