Effect of buyang huanwu tang and its disassemble formulas on the levels of superoxide dismutase,malondialdehyde and nitric oxide in serum and brain tissue in cerebral ischemia reperfusion models of rats

Yunke ZHANG

ABSTRACT

BACKGROUND:

There are various mechanisms on the occurrence of cerebral vascular disorders,in which,free radical and lipid peroxidation have participated in the process of nerve cell injury after cerebral ischemia. Buyang huanwu tang is a kind of commonly used Chinese herb for the treatment of ischemic cerebral vascular disorders,but its mechanisms have been unknown.

OBJECTIVE:

To observe effect of buyang huanwu tang and its disassemble formulas on the levels of superoxide dismutase(SOD),malondialdehyde (MAD) and nitric oxide(NO) in serum and brain tissue in cerebral ischemic reperfusion model in rats,probe into its mechanisms and study on the significance of formula compatibility.

DESIGN:

Experimental study with randomized control based on experimental.animals.

SETTING:

Neurological internal department of one hospital affiliated to an institution of Chinese medicine.MATERIALS The experiment was performed in 3rd Grade Experimental Room of State Administration of Traditional Chinese Medicine,Henan Academy of Chinese Medicine. Sixty clean-grade SD rats were employed in the experiment,provided by Shanghai SIPPR/BK Experimental Animal Co. Ltd.

INTERVENTIONS:

Sixty rats were randomized into sham-operation group,model group,huangqi(Radix Astragali seu Hedysari) group and general formula group. The model of cerebral ischemic reperfusion in SD rats were prepared by four vessel ligature method,in which,the medicine was applied at the dosages of 8 g/kg,40 g/kg and 48 g/kg in blood activation group,huangqi(Radix Astragali seu Hedysari) group and general formula group successively; and physiological saline 20 mL/kg was applied in both sham-operation group and model group. Serum and homogenate in brain tissue were collected to assay SOD with xanthine oxidase method,MAD with thiobarbituric acid method and NO with nitrate reductase method.MAIN OUTCOME MEARSURES Levels of SOD,MAD and NO in serum and brain tissue of rats in every group.

RESULTS:

The levels of MAD and NO in serum and brain tissue in model group were remarkably increased(P ＜ 0.001-0.05) and SOD was remarkably decreased(P ＜ 0.05,P ＜ 0.01) .In general formula group,huangqi (Radix Astragali seu Hedysari) group and blood activation group of disassemble formulas,the levels of MAD and NO were reduced and SOD was increased,for which,the action of general formula group was the strongest ( P＜ 0.01).

CONCLUSION:

Buyang huanwu tang and disassemble formulas act on being against the cerebral ischemic reperfusion injury achieved by anti-free radical.