Polymerase Chain Reaction Detection for Porcine Endogenous Retrovirus in 4 Pig Cell Lines / 中国兽医学报
Chinese Journal of Veterinary Science
;
(12): 1-3, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-410131
ABSTRACT
Porcine endogenous retroviruses (PERV) can infect human cell in vitro, which raised widely concerns re-garding the transmission of PERV to xenograft recipients. It's essential to establish a method for detection of PERV.3 pairs of primers were synthesized according to the sequence of gag, pol and env gene of PERV. Polymerase chainreaction (PCR) and reverse transcription PCR (RT-PCR) assays were performed for detection of PERV provirusDNA and PERV specific mRNA. The results showed that provirus DNA and mRNA of PERV existed and expressedin all 4 tested cell lines. The sizes of amplified fragments are identical with the predicted. These methods may be suit-able for monitoring PERV in other cells or tissue.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Veterinary Science
Year:
2003
Type:
Article
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