Detection of anti-Sm antibodies in SLE serum by immuno-PCR / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 263-265, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410406
ABSTRACT
Objective:
To apply immuno-PCR for detection of anti-Sm antibodies.Methods:
Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe. The experimental conditions were optomized and methological identifieation indicated obvious in crease(×107 ) in sensitivity compared with that of ELISA. The positive rate of anti-Sm antibodies was 54.9% in SLE sera, and significantly higher than that of ELISA. Intra-and inter-CV were 2.3%~4.8% and 3.7%~6.9% respectively. Immuno-PCR was positively correlated with ELISA( p <0.001). Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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