Determination of Cancer Antigen 15-3 Using an Activated Polymer Membrane Immunoelectrode / 分析化学
Chinese Journal of Analytical Chemistry
;
(12): 383-386, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410557
ABSTRACT
A potentiometric immunoelectrode with non-labeled anticancer antigen CA 15-3 antibody used for determinating CA15-3 was reported. firstly, the anti-CA15-3 antibody was immobilized on the surface of polyvinyl chloride-bovine serum albumin membrane, which was activated previously. Secondly, CA15-3 was added on the surface of the antibody membrane to form the antigen-antibody complex. Lastly, the complex membrane was used to assemble an immunoelectrode with an Ag-AgCl inner electrode. The potential of the complex membrane was measured with a pH meter. The response was linear over the range of 15~240 U/mL with a correlation coefficient of 0.9998. Other common antigens in human serum did not interfere with the determination of CA15-3. The mechanism of potential response was explored.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Analytical Chemistry
Year:
2001
Type:
Article
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