Determination of content of astragaloside A in Astragalus membranaceus powder for / 中草药

ABSTRACT

To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods　TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results　The regression equation within the spotted range of 80～240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion　The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.