EFFECT OF LIDAMYCIN ON THE EXPRESSION OF GENES INVOLVED IN INVASION REGULATION IN HCT-8 HUMAN COLON CANCER CELLS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 246-249, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410649
ABSTRACT
AIM To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. METHODS HCT-8 human colon cancer cells were treated with lidamycin (10 nmol*L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays, Northern blot and RT-PCR. RESULTS Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR. CONCLUSION The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2001
Type:
Article
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