Determination of astragaloside in serum with anisic acid by spectrophotofluorimetry / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6): 274-276, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410688
ABSTRACT
Objective:
To develop a new determination method of astrogaloside in s erum by spectrophotofluorimetry.Methods:
The fluorescence char acteristics of astragaloside anisic reaction product were used to determine astragaloside in serum. Re sults The reaction product of astrogaloside and anisic acid had excitatio n and emission maxima were at 320 and 387 nm, respectively. The linear range of determ inati on was between 0.2 and 40.0 mg*L -1. The detection limit was 0.02 mg*L -1. The recovery of measurement was between 98.8% and 102.4%.Conclusion:
The propos ed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine astragaloside in serum successfully.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Peking University(Health Sciences)
Year:
2001
Type:
Article
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