Effect of rapamycin on allograft rejection and immune response in mice / 北京大学学报(医学版)

ABSTRACT

Objective:To investigate the effects of rapamycin on allograft rejecti on and immune response in mice. Methods:The heterotopic ear-hea rt grafting or sk in grafting were done in mice. The delayed type hypersensitivity (DTH) response i nduced by dinitro-fluorobenzene (DNFB), the production of hemolysin of mouse sensitized by sheep red blo od cell (SRBC) and the neutral red phagocytosis of the peritoneal macrophage wer e also tested in mice. Results:RAPA significantly blocked allograft reject ion of heart and skin, markedly inhibited DTH response and decreased the production of hemolysin,but had no significant effect on the neutral red phagocytosis of the p eritoneal macrophage. Conclusion:RAPA potently blocked allograft rej ections in mi ce and suppressed cellular and humoral immune response, but had no significant e ffect on phagocytoses of macrophage.