Detection polymorphism of CYP1A1 gene by allele specific amplification-ELISA / 临床检验杂志

ABSTRACT

Objective For establishing a effective method of gene polymorphism. Methods Polymorphism of CYP1A1 were detected by allele specific amplification and allele specific amplification-ELISA in this study.Results The results of two testing methods were same except one sample.PCR products were labeled with Dig in ASA-ELISA,then the PCR products were detected by specific and sensitive ELISA.The assay detection was 100-fold more sensitive than gel detection of ASA PCR products.Conclusion It was simple and short time,but it was more expensive.