ffect of basic fibroblast growth factor on rabbit lens epithelial cell proliferation / 眼科新进展
Recent Advances in Ophthalmology
;
(6): 161-163, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410892
ABSTRACT
Objective To study the effect of basic fibroblast growth factor (bFGF) on proliferating of rabbit lens epithelial cells(RLECs).Methods The second and three generations of RLECs were exposed to different concentrations of bFGF, and the proliferation characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay. The ultrastructure of cell were observed with transmission electron microscope (TEM).The changs of cells cycles were observed with flow cytometer (FCM).Results After the treatment of bFGF, RLECs showed the marked proliferation , especially in 10μg*L-1 of bFGF . TEM result showed that the cells were more active with bFGF, FCM result showed that the S phase cell obviously increased.Conclusion bFGF is an important factor that can promote proliferation of RLECs.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Recent Advances in Ophthalmology
Year:
2001
Type:
Article
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