Effect of angiotensin II on the three transcription factors in endothelial cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 196-199, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410946
ABSTRACT
AIM and METHODS:
The effects of angiotensin II(AngII) on the activation of three transcription factors were investigated by using EMSA and western-blot methods in endothelial cells respectively.RESULTS:
The EMSA results showed that AngII stimulation could increase NF-κB, SP-1 and AP-1 activation in ECV304, which suggested that activity changes in these three transcription factors could partly contribute to the dysfunction of endothelial cells.The binding affinity of NF-κB, SP-1 and AP-1 with corresponding oligonucleotides in AngII-treated ECV-304 were respectively 10.98,3.89,1.33 times as large as in control. The nuclear appearance of AngII-activated NF-κB was examined by western-blot, which corroborates our results from EMSA analysis. While as the protein appearance of AP-1 and SP-1 in nucleus, were little higher than the control group. The result of western-blot suggested that AngII-induced EC activation of these three transcription factors maybe mainly due to the enhanced binding ability to its corresponding cis-acting factors.CONCLUSION:
NF-κB, a ubiquitously exposed nuclear transcription factor, is involved, together with SP-1,AP-1, in the regulation of endothelial cell dysfunction related to cardiovascular diseases such as atherosclerosis and hypertension.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2001
Type:
Article
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