Study on antisense oligoncleotides as inhibitor of human acute promyelocytic leukemia proliferation and protein expression / 中国新药与临床杂志
Chinese Journal of New Drugs and Clinical Remedies
;
(12): 174-176, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410978
ABSTRACT
AIM:
To study on antisense oligoncleotides as inhibitor of human acute promyelocytic leukemia (HL-60) proliferation and C-myc protein expression.METHODS:
Oligonucleotides with different lengths (18-21 mer) complementary to the definite regions of C-myc mRNA, modified groups (with S replaced O in internucleotide phosphate linkage) and unmodified ones (with natural internucleotide phosphate linkage) were designed and synthesized. These olignucleotides were tested for their activity on HL-60 cell and also for their toxicity on normal lymphatic cells of human.RESULTS:
It was found that two of the oligonucleotides complementary to 5′-44-61 and 5′-556-576 the regions of C-myc mRNA exhibited great inhibitory effects (59.5 % and 62.7 %) on growth of HL-60 cells for a definite time. And no toxicity of the two antisense oligonucleotides was found on normal lymphatic cells of human.CONCLUSION:
The sequence of antisense oligonucleotides complementary to 5′-44-61 of C-myc mRNA was designed newly by us may be turned into inhibitory medicine of HL-60 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of New Drugs and Clinical Remedies
Year:
2001
Type:
Article
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