The relationship between the expression of Cx43 and the proliferation of keratinocytes in epidermis of rats / 北京大学学报(医学版)

ABSTRACT

Objective： To study topographic characters of the expression of Cx43 and the proliferation activity of keratinocytes on the upper legs of rats and to explore the relationship beween them. Methods： By rutine paraffin section , immunohistochemical techniques of Cx43 and PCNA were used to study the length of the expression of Cx43 in a unit area of epidermis(LA) and the percentage of proliferating cell nuclear antigen（PCNA）positive nucleus(%). Results： Within the range of 5 mm in the horizonal direction, the expression of Cx43 in epidermis varied according to the locations of keratinocytes and Cx43 was highly expressed which was about 3.0～4.5 mm from the start point. At the same time, within the range of 10 mm in the horizonal direction, the proliferation activity of keratinocytes was different from site to site and the kerationcytes with similar proliferation activity were clustered as groups, the scale of which was about 10－3 m; One of the sites, with low proliferation activity, was about 3.0～4.0 mm from the start point. Conclusion： This study suggested that the keratinocytes with low proliferation activity had high expression of Cx43.