bcl-xs mediated sensitization of human nasopharyngeal carcinoma cells to camptothecin-induced apoptosis / 中国耳鼻咽喉头颈外科
Chinese Archives of Otolaryngology-Head and Neck Surgery
;
(12): 114-117, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411187
ABSTRACT
Objective:
In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:
bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:
Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:
Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Archives of Otolaryngology-Head and Neck Surgery
Year:
2001
Type:
Article
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