Separation, Purification and Analysis of the Components of Earthworm Fibrinoly tic Enzymes / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
;
(12): 218-221, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411259
ABSTRACT
The earthworm fibrinolytic enzymes (EFE) were separated by affinity ch romatography using soybean trypsin inhibitor as a matrix. The enzymes were furth er separated and purified into 12 components after DEAE-32 chromatography and p reparative electrophoresis. The pI of these components gradually decreased f rom pH 4.0 according to electrophoresis mobility from higher to lower on PAGE. The molecular weights were in the range of 22~34 ku. 6.5 and 7 w ere glycoproteins proved by staining with the shiff reagent and thymol/sulfuric acid. The fibrinolytic activity of 7 was highest as determined usi ng chromzym UK and chromzym PL as specific substrates.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Progress in Biochemistry and Biophysics
Year:
2001
Type:
Article
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