The study of the expression of the antisense Ku70 in LCA and its radiosensitivity / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 82-84, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411357
ABSTRACT
Objective:
To study the expression of the antisen se Ku70 in a human's lung carcinoma cell line(LCA) and its high-radiosensitivit y.Methods:
The expression of the Ku70 protein in the Ku70- LCA and its radiosensitivity was detected by Western-blott ing and Cell Radiosensitivity Taste (to see The Cell Clonogenic Assay and Surviv al Curves).Results:
(1)Only the sense Ku70 protein was defe cted in the Ku70-LCA,but the sense Ku70 protein wasn't defected in the Ku70as -LCA by Western-blotting.(2)The radiosensitivity was increased in the Ku70as- LCA and their radiosensitivity depend on the radiating dose(P<0.01).Conclusion:
After the expression of sense Ku70 protein in t he Ku70as-LCA was blocked by antisense Ku70,the Ku70as-LCA should be high-rad iosensitivit y,the Ku70 is a candiate target for cancer gene therapy.It was the first step fo r the study of tumor's specific radiating and gene therapy in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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