Determination of floxuridine in plasma by HPLC / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
;
(6): 84-85, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411419
ABSTRACT
Purpose The aim is to establish the HPLC method for the determination of Fluoro-deoxyuridine in plasma.Methods The Chromatography conditions include Chromatography column Nova-pak C18(3.9mm×150mm,4μm), mobile phase 0.05mol/L sodium phosphate monobasic -methanol-water(0.5∶7∶92.5), UV detection at 260nm, FUDR was extracted with ethyl acetate. Results The average recoveries were 96.4%,96.5%,97.8% for concentration 0.23、1.67、20.0μg/ml (n=5).The corresponding reproducibility were RSD 1.61%, 1.98%, 3.17% respectwely for iner-day and RSD 3.56%, 1.90%, 2.63% for the intra-day(n=5). The FUDR concentration was linear with a correlation coefficient of 0.999 4 over the range of 0.099~20.0μg/ml. Conclusion The method was sensitive and accurate and suitable for pharmacokinetics and bioavailability study of FUDR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Biochemical Pharmaceutics
Year:
2001
Type:
Article
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