Purification of human urine erythropoietin and thrombomodulin / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
; (6): 57-60, 2001.
Article
in Zh
| WPRIM
| ID: wpr-411428
Responsible library:
WPRO
ABSTRACT
Purpose The aim is to isolate and purify the erythropoietin(EPO)and thrombomodulin(TM) from human urine.Methods Purifying and isolating human urine with means of filtration and concentration,ion-exchange chromatography, affinity chromatography and so on.Results 4.47 mg EPO and 9.92 mg TM were obtained from 2 000 kg human urine.Their molecular weights were(38.6±1.0)kD and (60±1.4)kD erespectively, the isoelectric point, 3.60±0.02 and 3.70±0.02, yield, 31.9% and 25.0%.The purified EPO and TM was shown to be highly homogeneous by capillary zone-electrophoresis(CZE),abundant Glu,Ala and Gly include on TM. Conclusion The high purity EPO and TM was obtained.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Biochemical Pharmaceutics
Year:
2001
Type:
Article