The Effect of Salvia Miltiorrhiza on Gentamicin Ototoxicity-Induced Activity of Nitric Oxide Synthase in Cochlea of Guinea Pig / 中国医科大学学报

ABSTRACT

Objective: Our purpose was to explore the protective role of Salvia Miltiorrhiza (SM) on gentamicin (GM) ototoxicity. Methods: We used NADPH-diaphorase in histochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement to investigate the change of nitric oxide synthase (NOS) activity in cochlea of guinea pig after the injection of GM and SM. Results: Gebtamicin and SM significantly reduced NOS activity in cochlear and the threshold of ABR compared with GM alone (P<0.01); and the threshold of ABR was in high correlation with NOS activity (rGM= －0.8236 ～ －0.8662; rSM+GM= －0.8628 ～ －0.9172, P<0.01). Conclusion: Salvia Miltiorrhiza can reduce NOS activity in cochlea, alleviate GM ototoxicity, and ameliorate hearing function.