Study on cloning of the Egr-1 promoter and its radiation-inducible property / 吉林大学学报(医学版)

ABSTRACT

Objective:Egr-1 promoter was amplificated and Egr-PGL plasmid was constructed to study the expression of luciferase in transfected NIH3T3 cells after different doses of X-ray irradiation.Methods:Egr-1p was amlificated by PCR and inserted into PGL3-E vector.The expression of luciferase induced by X-ray was studied by counting the light of luciferase and stubstrate.Results:Egr-1 cDNA was obtained by PCR and was sequenced.The results indicated the sequence was almost correct.The Egr-1p was connected with PGL3 vector and was detected by electrophoresis.The constructs were used to transfect mouse NIH3T3 cells to characterize the regulatory function of Egr-1p after exposure to X-ray irradiation.The results indicate that the expression of luciferase of all groups irradiated is highter than that of 0 Gy group.The expression of groups irradiated is about 5-7.5 times greater than that of 0 Gy group.Conclusion:Egr-1pobtained can induce the expression of its downstream gene after different doses of X-ray irradiation.Low dose irradiation also can do it and it is may be more important in tumor gene therapy.