Effect of human growth hormone on cell cycle kinetics in colonic cancer cells / 肠外与肠内营养
Parenteral & Enteral Nutrition
;
(6): 8-10, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411627
ABSTRACT
Objectives:
The effects of human growth hormone (hGH) on cell cycle kinetics were studied in human colonic cancer cell line, LS-174-T cell.Methods:
The cells were cultured in RPMI-1640 medium for 24 hours with hGH at concerntration of 50, 100, 200 ng/ml with or without cisplatin. Cell cycle kinetics was analyzed by flow cytometry.Results:
The significant effects of hGH on cell cycle kinetics were found in the cell line. Percentage of S phase increase (P<0.01) and percentage of G2/M phase decreased (P<0.01).DNA Index (DI) didn't increase.The cells had a lot of apoptosis and percentage of S phase decreased (P<0.01) with addition of cisplatin.Conclusions:
In vitro hGH may induce the proliferation of colonic cancer cells. Cisplatin may suppress the stimulatory effects of hGH on S phase and increase the apoptosis of colonic cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Parenteral & Enteral Nutrition
Year:
2001
Type:
Article
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