Effect on D1-Like and D2-Like agonist on GABAA-receptor in rat dorsal root ganglion / 基础医学与临床
Basic & Clinical Medicine
;
(12): 36-39, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411665
ABSTRACT
The experiment was performed on neurons freshly isolated from rat DRG by using whole cell patch clamp techniques. When 60 cells responded to GABA were treated with SKF38393, a selective agonist of dopamine Dl receptor, it was found that except one cell which exhibited a slight potentiation( l0.0%,l/60), the inhibition by SKF38393 of GABA-activated current was predominant (88.33%,53/60), while the remainders were of no effect( l.66%,6/60). In 50 neurons tested , when the neurons were treated with R(-)-NPA prior to the application of GABA for 30sec, It was found that GABA-activated membrane currents were inhibited (76%,38/50) , enhanced (2%,l/50) , no effected markedly (22%,11/50). The effect of SKF38393 and R(-)-NPA was dependent on the concentration of SKF38393 and R(-)-NPA . By intracellular application of 10-4mol/L H7, a potent inhibitor of protein kinase , via micropipette the inhibition of SKF38393 and R(-)-NPA was abolished apparently as compared with the control .
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2001
Type:
Article
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