Detection of IgG2b Monoclonal Antibodies Against LDH-C4 in Sera of Mice Bearing Hybridoma Backpack Tumors by Quantitative ELISA / 昆明医科大学学报
Journal of Kunming Medical University
;
(12): 30-33, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411706
ABSTRACT
The levels of IgG2b monoclonal antibodies against LD H-C4 in sera of mice bearing hybridoma backpack tumors secreting anti-LDH-C4-IgG2b were detected by quantitative ELISA. The accuracy between batches is 7.04%~l3.30 %, the intra-assay variation is 3.6l%~l0.20%. Standard curveof monoclonal lgG2b was well correlated (r=0.962 884~0.996 795). The sensitivit y of the assay reach e dup to0.0l?mg/L. The present modified ELISA offers a reproducible, se nsitive, specific method in determination of antigen-specific IgG2b antibody in sera.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Kunming Medical University
Year:
2001
Type:
Article
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