The Study on Improving Method of Extracting Bacteria Plasmid / 昆明医科大学学报
Journal of Kunming Medical University
;
(12): 42-44, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411709
ABSTRACT
Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity. Result The eight strip s of Ecliv 5l7 can not be found completely in original method, but can be found in our one.We found the largest plasmid molecular weight was l86.3?kb, and t he minimum was l.l?kb from each 30 strains STA, EC, PA. Plasmid profile is alike through repeating ex p eriment three times. Conclusion:
The method can be widely used in extracting bac teria plasmid and fit gram-negative and gram-positive bacteria whenever molecular weight ma x or min. These conditions can be obtained in general laboratory.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Kunming Medical University
Year:
2001
Type:
Article
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