Transfection and expressiOn of green fluorescent protein gene in prostate carcinoma PC-3M cell line / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 592-594, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-411794
ABSTRACT
Objective:To reseach the expression of green fluorescent protein(GFP)gene in prostate car-cinoma PC-3M cell line and the efffect of cell cycle.Methods:GFP gene recombination DNA was construct-ed and PC-3M cells were transfected with lipofectin regant.The expression of GFP was observed under in-vert fluorescence microscope.Meanwhile,the cell cycle of positive cell colon was assayed by flOw cytome-ter.Resuits:After transfection,about 10%~15%cells expressed green fluorescent protein and there wasno significant change compared the cell cycle of positive cell colon with parents cells.Conclusion:GFP sup-plys a new way in reseaching metabolic phenomenon of live cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2000
Type:
Article
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