Study on targeting therapy of idiopathic thrombocytopenic purpura with MTX-labelled antibody in vitro / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 579-581, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-411798
ABSTRACT
Objective:
To conjugate IVIG and MTX to produce a specific cytotoxicity upon phagocytes.Methods:
MTX was conjugated with IVIG by indirect conjugating methods. HSA was used as an intermedi-ary to conjugate MTX with IVIG. The indirect immunofluorescence was adopted to test the binding abilityof Fc fragment. MTT assay was used to measure the cytotoxicity of conjugation on phagocytes.Results:
Conjugation showed stronger cytotoxicity upon target cells than free MTX,and it showed only less cyto-toxic effect on Fc receptor negative cells compared with the positive ones. The specific cytotoxicity of IVIG-HSA-MTX was significantly stronger than that of MTX.Conclusion:
In vitro the conjugation showed ahighly specific cytotoxicity upon phagocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2000
Type:
Article
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