Expression of nm23-H1 gene in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the variation of nm23-H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23-H1 expression and OSCC metastasis to neck lymph nodes.Methods:nm23-H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis;nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results： All samples showed no change in nm23-H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23-H1 mRNA,however,only 5 were found no nm23-H1 mRNA in 21 cases without metastasis,positive rate of nm23-H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23-H1 gene showed significant negative relationship with neck lymph nodes metastasis (P<0.01);no relationship was found between expression of nm23-H1 gene and cancer pathological classification.Conclusion:The change of nm23-H1 expression doesn't result from the variation of gene structure;the product of nm23-H1 gene probably inhibit OSCC metastasis to neck lymph nodes.