A new method for the determination of the activity of HGF / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
;
(6): 10-12, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-411952
ABSTRACT
Purpose The aim is to develop a new method for the determination of the activity of hepatocyte growth factor (HGF). Met hods Rat hepatocytes were prep ared by collagenase perfusion and incubated at 5% CO2 incubator for 1.5 h. The n HGF was added into the culture medium and incubated at 5% CO2 incubator for 1.5 h. The cell viability was determined by MTT method.Results ~1 142 μg/ml improved the number of cell viability significantly (AHGF/Acontrol) >2.Conclusion Th is new method was stable, time-saving and of good reappearance. It was adapted to the determination of HGF activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Biochemical Pharmaceutics
Year:
2001
Type:
Article
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