Infection of peripheral blood mononuclear cells by hepatitis C virus and the influ-ence on T lymphocyte subset / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 468-470, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-412268
ABSTRACT
To investigate peripheral blood mononuclear cells (PBMCs) infected by hepatitis C virus (HCV) and the influenceon T lymphocyte subset. Methods:
Using non-isotopic in situ hybridization (NISH) and streptadivin-biotin-enzyme complex assay (SABC) todetect HCV-RNA, NS5 antigen and T lymphocyte subset.Results:
8(40.0% ) out of 20 patients with hepatitis C virus were HCV-RNA posi-tive in PBMCs, and among them 6(30.0%) were NS5 antigen positive simultaneously. NS5 antigen signals appeared diffusely within cyto-plasm, or in cell membrane, while HCV-RNA signals were mainly distributed in cytoplasm. Both the proportion of NS5 positive cell and HCV-RNA positive cells were very low (≤3%).The proportion of positive cellsane, while HCV-RNA signals are very low (≤3%). The proportionof CD4+ T cells was lower in the patients comparing with that in control group, the proportion of CD8+ T cells was higher, and the ratio ofCD4+/CD8+ were decreased remarkably (P<0.01). Moreover the proportion of CD4+ T cells was lower in HCV-RNA-positive group com-paring with that in HCV-RNA-negative group, the proportion of CD8+ T cells was higher, and the ratio of CD4+/CD8+ was reversed (P<0.01).Conclusion:
HCV can infect PBMCs and replicate in patients with chronic hepatitis C;CD4+ T cells are readily damaged after PBMCsinfected by HCV, which causes the decrease or reversal of the ratio of CD4+/CD8+, undermines the ability of the body clearing virus and re-sults im chronicity of HCV infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Type:
Article
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