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Binding Properties of M-CAT Element in the Chicken Nicotinic Acetylcholine Receptor γ-Subunit Promoter / 中国生物化学与分子生物学报
Chinese Journal of Biochemistry and Molecular Biology ; (12): 580-585, 2000.
Article in Chinese | WPRIM | ID: wpr-412335
ABSTRACT
Several M-CAT elements are present in the chicken nicotinic acetylcholine receptor γ-subunit promoter and required for the totipotency of this gene.To understand the binding properties of M-CAT element,the interaction of M-CAT motif,at the position -67/-61 relative to the initiation site of γ-promoter with nuclear proteins,were analyzed.Mutageneses and binding experiments demonstrated that the M-CAT core motif bound nuclear protein (s) from tested chicken tissues to generate a high-mobility complex;while the flanking sequence bound different factors to generate lower-mobility complexes with the exception of liver.Southwestern blotting showed that only a 30 kD protein was expressed in tested tissues of chicken embryo at day 13.These results suggest that a 30 kD factor can directly bind to M-CAT element in the manner of monomer,even homodimer and is ubiquitously expressed in tissues,while the binding of the factor(s) to flanking sequence should probably rely on the presence of a 30 kD ubiquitous factor.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Biochemistry and Molecular Biology Year: 2000 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Biochemistry and Molecular Biology Year: 2000 Type: Article