Effects of different intensities and radiation durations of microwaves on activation of extracellular-regulated kinase (Erk)-1/2 in cultured mouse fibroblast / 中华物理医学与康复杂志

ABSTRACT

Objective To investigate the effects of different intensities and different radiation durations of 2450MHz microwaves on activation of extracellular-regulated kinase (ERK)-1/2 in cultured mouse fibroblast in vitro.Methods The fibroblasts isolated from mouse skin and cultured were directly radiated with different intensities and different durations of microwave.After direct radiation with 2450MHz microwave (0.3 W/cm2, 0.5W/cm2, 1W/cm2 ) for 15min or 30min, anti-phosphorylation extracellular-regulated kinase( ERK-1/2 ) antibody was analyzed by Western blot technique to observe phosphorylation changes of fibroblasts protein ERK-1/2.and activation of mitogen-activated progein kinase(MAPK) signal conductive pathways.Results After microwave radiation with 0.3W/cm2, 0.5W/cm2, 1W/cm2for 15min, activation of ERK-1/2 increased significantly (P < 0.05 ) ; but after 30min radiation with 0.3 W/cm2,0.5W/cm2, 1W/cm2, activation of ERK-1/2 decreased significantly(P<0.05).Conclusion It is concluded that different intensities microwave may activate different signals of fibroblasts in dose-dependent manner in vitro.Different microwave radiations can induce different activations in MAPK signal conductive pathways.