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The study on SDF-1α and c-MYC protein regulate tachykinin receptor 1-trucated mRNA expression / 中华微生物学和免疫学杂志
Article in Zh | WPRIM | ID: wpr-412522
Responsible library: WPRO
ABSTRACT
Objective To investigate how SDF-1α and c-MYC protein regulates TACRl-Tr expression. Methods c-myc shRNA vector was constructed, small interfering RNA was employed for silencing c-myc gene in MCF-7 breast cancer cell. SDF-1α neutralized antibody was used in c-myc+ cell group and c-myc- cell group, while other c-myc+ cell group and c-myc- cells group were cultured under normal condition. The mRNA level of TACRl-Tr was determined by real-time PCR. Results c-myc shRNA vector was constructed successfully, in the normal presence of SDF-la, the level of TACRl-Tr mRNA in c-myc- cell group were lower than that in c-myc+ cell group( P < 0.05). But in the presence of SDF-la neutralized antibody, TACRl-Tr mRNA level of c-myc- cell group was higher than that of c-myc+ cell group(P < 0.05). Conclusion In the normal culture condition, c-MYC protein may transactivate TACRl-Tr transcription in MCF-7 cell, in the presence of SDF-1α neutral antibody, c-MYC protein lost the activity of transactivating for TACRl-Tr transciption.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Microbiology and Immunology Year: 2011 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Microbiology and Immunology Year: 2011 Type: Article