Protective Effects of Epigallocatechin Gallate After UV Irradiation of Cultured Human Lens Epithelial Cells
Korean Journal of Ophthalmology
;
: 183-186, 2008.
Article
in English
| WPRIM
| ID: wpr-41299
ABSTRACT
PURPOSE:
To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells.METHODS:
We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.RESULTS:
Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells.CONCLUSIONS:
EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Injuries
/
Radiation-Protective Agents
/
Tetrazolium Salts
/
Thiazoles
/
Ultraviolet Rays
/
Catechin
/
Cell Count
/
Cell Survival
/
Cells, Cultured
/
Dose-Response Relationship, Drug
Limits:
Humans
Language:
English
Journal:
Korean Journal of Ophthalmology
Year:
2008
Type:
Article
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