Effects of trehalose on bcl-2 and bax mRNA expression in the cryopreserved sternum / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 3975-3978, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-415349
ABSTRACT
BACKGROUND:
Studies have demonstrated that trehalose possesses protective effects on cyropreserved sternum. But the mechanism of action remains poorly understood.OBJECTIVE:
To investigate the effects of trehalose on bcl-2 and bax mRNA expression in cryopreserved sternum.METHODS:
Four groups of freshly prepared solution were used low-potassium dextran (LPD), LPD + dimethyl sulfoxide (DMSO), LPD + trehalose, LPD + DMSO + trehalose. Rat sternum was cut and then immediately cryopreserved in the tubes containing each group of solution. Fresh rat sternum tissue and 4 groups of samples cryopreserved for 120 days were taken and bcl-2 and bax mRNA expression in fresh and cryopreserved sternum was detected using reverse transcription-polymerase chain reaction.RESULTS ANDCONCLUSION:
bcl-2 mRNA expression in the LPD + trehalose group was significantly higher, but bax mRNA expression was significantly lower, than in the LPD, LPD + DMSO groups (both P 0.05). These findings indicate that trehalose may protect cell activity in cryopreserved sternum by enhancing bcl-2 mRNA expression and inhibiting bax mRNA expression, and trehalose together with DMSO shows better protective effects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2011
Type:
Article
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