Development of hepatitis C virus by fluorescent real-time reverse transcription loop-mediated isothermal amplification method / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 564-566, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-415657
ABSTRACT
Objective To establish a rapid, sensitive, and specific quantitative method to detect hepatitis C virus. Methods A primer set targeting HCV 5'UTR was designed. The isothermal amplification was performed by the Bst DNA polymerase and AMV reverse transcriptase, under the temperature of 60℃ for 60 min. The signal was monitored by SYBR Green Ⅰ. Results One hundred and twenty positive serum samples, confirmed by the real-time PCR. All were detected by the isothermal amplification, while 110 healthy subjects' samples were negative by the both methods. The lower detect limit was determined to 10 IU/ml HCV-RNA, by the assay on serial dilutions of the quality control standards obtained from clinical investigation center of MOH. Conclusion A real time reverse loop-mediated isothermal amplification method was developed to detect HCV, with the characteristic of rapidity, high sensitivity and specificity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Practice guideline
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2011
Type:
Article
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