Effects of the serum of rats administered rutin on the proliferation, differentiation and maturation of rat calvarial osteoblasts in vitro / 国际中医中药杂志

ABSTRACT

Objective It was studied that the effects of the serum of rats administered rutin on the proliferation, differentiation and maturation of rat calvarial osteoblasts in Vitro. Methods Osteoblasts were obtained from the newborn calvaria of Wistar rats younger than 24 hrs. After the bone pieces were digested with collagenase-trypsin, osteoblasts were released and were fed with α-MEM containing 10% fetal bovine serum. 12 adult Wistar rats were given rutin intragastrically at 25 mg/100 g body weight and their serum was obtained after 1hour. The control serum was obtained by giving equal volume of physiological saline. The serums were supplemented into the culture media of rat calvarial osteoblasts by 2.5%, 5% and 10% respectively. The proliferation of ROB was analyzed by MTT reduction assay. The osteogenic differentiation and maturation were assayed by measuring alkaline phosphatase activity(ALP)and numbering the mineralized bone nodules. Results The value of A570 in 2.5% and 5% SR group were Significantly [(0.678±0.242)%、(0.796±0.155)%]higher than those of the control. 10% SR was toxic to ROB. 5% SR was stronger (63.35±5.20)μg/μl than 2.5% SR in increasing ALP activity and the number of mineralized bone nodules which were significantly higher in SR group than in the control. Conclusion The metabolites of rutin after oral administration stimulate the proliferation and osteogenic differentiation of osteoblasts. Because so far, the vast majority of the Chinese herbal medicine containing Rutin are oral. The metabolites of rutin after oral administration are likely to be the effective in anti-osteoporosis. However, the role of specific forms and modalities have yet to be studied in depth.