Effects of tacrolimus on the expression of stem cell factor(SCF)mRNA in human HaCaT keratinocytes and c-kit mRNA in murine B16 melanoma cells / 中华皮肤科杂志
Chinese Journal of Dermatology
;
(12): 509-510, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-416990
ABSTRACT
Objective To investigate the effects of tacrolimus on the expression of SCF mRNA in keratinocytes and c-kit mRNA in melanoma cells.Methods Tacrolimus was used to treat cultured HaCaT keratinocytes and B16 melanoma cells for 48 hours.Subsequently,real-time fluorescence quantitative PCR was performed to determine the mRNA expression of SCF in HaCaT cells and of c-kit in B16 cells.Results A significant change was observed in the expression of SCF mRNA in HaCaT cells and c-kit mRNA in B16 cells treated with tacrolimus compared with untreated HaCaT cells and B16 cells,respectively (both P<0.05).Conclusion Tacrolimus can upregulate SCF mRNA expression in HaCaT cells and c-kit mRNA expression in R16 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Dermatology
Year:
2011
Type:
Article
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