Expression of bradykinin as a substrate of CD26/DPP Ⅳ in rats ischemia/reperfusion injury following lung transplantation / 中华器官移植杂志

ABSTRACT

Objective To investigate the expression of bradykinin as a substrate of CD26/DPP Ⅳ in rats with ischemia/reperfusion injury following lung transplantation (LTx). Methods Thirty-six syngeneic male SD rats were randomly allocated into control group and experimental group (n= 18each), and 36 rats served as donors. In control group and experimental group, the lungs were flushperfused and preserved with LPD and LPD+ CD26/DPP TV catalytic inhibitor (AB192) respectively,and LTx was performed 18 h after cold ischemia Histopathological studies were also done on the same locus of lung specimens and staining for bradykinin. The different proteins were separated by means of immobilized PH gradient-based two-dimensional gel electrophoresis. The differential expression of bradykinin was compared by Western blotting. Results The expression of bradykinin in control group was significantly higher than in experimental group. As compared with experimental group,bradykinin expression was significantly up-regulated in control group (P <0 . 05 ). Conclusion Inhibitor of CD26/DDP Ⅳ enzymatic activity significantly ameliorated ischemia/reperfusion injury in the early stage after LTx, which is probably associated with inhibition of the protein expression of bradykinin.